autoMACS Pro产品说明
产品概述
   
autoMACS 是一个计算机控制的、可高速、高纯度、高回收率地分选多种类型细胞的全自动磁性细胞分选器。该仪器基本原理是MACS磁性细胞分选技术，自动进行细胞分选过程，包括预冲分选柱、样本摄取、洗脱阳性成分和阴性成分、冲洗、清洗和分选柱的维护。autoMACS Pro是一个开放的技术平台，广泛应用于细胞生物学、免疫学、肿瘤学、血液病学研究。
分选后细胞可直接用于后续实验：流式细胞术检测、分子生物学研究、回输给动物、以及功能研究，例如：肿瘤细胞的纯化、肿瘤疫苗研制、干细胞技术、免疫治疗的基础研究、异基因干细胞移植后的系特异性嵌合体分析、HLA分型和HIV研究。
 
技术原理：
MACS（磁性细胞分选技术）是一种广泛用来磁性分选多种细胞或者生物分子的技术。
• 基于抗体对抗原的特异性识别
 
• 磁性微珠直接或者间接偶联在抗体上，从而与细胞相连
 
• 在高强度、梯度磁场中达到细胞磁性分离的目的
 
与相应抗体偶联的MACS微珠可以磁性标记目的细胞，然后通过一个高强度和梯度的磁场，磁性标记细胞滞留在磁场中，而未标记细胞流出。去除磁场后，磁性标记细胞可以被洗脱下来。磁性标记和未磁性标记成分均可以回收利用。
 
autoMACS技术指标：
1、MACS微珠直径50nm。
2、使用MACS分选柱进行分选。
3、MACS微珠能降解，对细胞无毒性，分选后的细胞仍保持生理功能。
4、与流式细胞仪兼容，分选后细胞可以立即用于分析和后续实验。
5、分选后细胞纯度高、回收率和回收率高。
6、可直接用全血进行分选。
7、标本量从105到4X109，进样量从0.2ml到50ml。
8、内置12个预设分选程序，一机完成阳性分选、去除分选、低频细胞分选、抗原弱表达细胞分选。可以分选任何种类细胞。




 
autoMACS pro部分参考文献
 
AutoMACS Pro在免疫学中的应用 

Perez, J. et al. (2011) Accumulation of CD4+ T cells in the colon of CsA-treated mice following myeloablative conditioning and bone marrow transplantation. Am. J. Physiol. Gastrointest. Liver Physiol. 300: G843–G852.

 
 

Bonnefoy, F. et al. (2011) TGF-β–exposed plasmacytoid dendritic cells participate in Th17 commitment. J. Immunol.186: 6157–6164.

 
 

Soroosh, P. et al. (2011) Herpesvirus entry mediator (TNFRSF14) regulates the persistence of T helper memory cell populations. J. Exp. Med. 208: 797–809.

 
 

Li, L. et al. (2011) Transcriptional regulation of the Th17 immune response by IKKα. J. Exp. Med. 208: 787–796.

 
 

Nanua S. et al. (2011) Activation of the unfolded protein response is associated with impaired granulopoiesis in transgenic mice expressing mutant Elane. Blood: 117: 3539–3547.

 
 

Sun J.C. et al. (2011) Homeostatic proliferation generates long-lived natural killer cells that respond against viral infection. J. Exp. Med. 208: 357–368.

 
 

Liu X.et al. (2011) High-dose dexamethasone shifts the balance of stimulatory and inhibitory Fc receptors on monocytes in patients with primary immune thrombocytopenia. Blood 117: 2061–2069.

 
 

Janikashvili N.et al. (2011) Allogeneic effector/memory Th-1 cells impair FoxP3+ regulatory T lymphocytes and synergize with chaperone-rich cell lysate vaccine to treat leukaemia. Blood 117: 1555–1564.

 
 

Sanz E. et al. (2010) Ordering human CD34+CD10–CD19+ pre/pro-B-cell and CD19– common lymphoid progenitor stages in two pro-B-cell development pathways. Proc. Natl. Acad. Sci. USA 107: 5925–5930.

 
 

Cai S.F. et al. (2010) Granzyme B is not required for regulatory T cell–mediated suppression of graft-versus-host disease. Blood 115: 1669–1677.

 
 
在肿瘤研究中的应用

Liu, Y. et al. (2011) Expression of p16INK4a prevents cancer and promotes aging in lymphocytes. Blood 117: 3257–3267.

 
 

Vargova, K.et al. (2011) MYB transcriptionally regulates the miR-155 host gene in chronic lymphocytic leukemia. Blood 117: 3816–3825.

 
 

Tawara, I.et al. (2011) Interleukin-6 modulates graft-versus-host responses after experimental allogeneic bone marrow transplantation. Clin. Cancer Res. 17: 77–88.

 
 

Tokunaga, M. et al. (2010) BCR-ABL but not JAK2 V617F inhibits erythropoiesis through the ras signal by inducing p21CIP1/WAF1. J. Biol. Chem. 285: 31774–31782.

 
 

Thompson, E.D. et al. (2010) Tumor masses support naive T cell infiltration, activation, and differentiation into effectors. J. Exp. Med. 207: 1791–1804.

 
 
在干细胞研究中的应用

Carlson, S. et al. (2011) Cardiac mesenchymal stem cells contribute to scar formation after myocardial infarction. Cardiovasc. Res. 91: 99–107.

 
 

Chen, T. H.-P. et al. (2011) Knockdown of Hspa9, a del(5q31.2) gene, results in a decrease in hematopoietic progenitors in mice. Blood 117: 1530–1539

 
 

Gieseke, F. et al. (2010) Human multipotent mesenchymal stromal cells use galectin-1 to inhibit immune effector cells. Blood 116: 3770–3779.

 
 

Shono, Y. et al. (2010) Bone marrow graft-versus-host disease: early destruction of hematopoietic niche after MHC-mismatched hematopoietic stem cell transplantation. Blood 115: 5401–5411.

 
 

Yamashita, T. et al. (2010) Oncostatin M renders epithelial cell adhesion molecule–positive liver cancer stem cells sensitive to 5-fluorouracil by inducing hepatocytic differentiation. Cancer Res. 70: 4687–4697.

 
 

Song, J.et al. (2010) An in vivo model to study and manipulate the hematopoietic stem cell niche. Blood 115: 2592–2600.

 
 

Gutman, J.A. et al. (2010) Single-unit dominance after double-unit umbilical cord blood transplantation coincides with a specific CD8+ T cell response against the non-engrafted unit. Blood 115: 757–765.

 
 
分子应用

Zhao, L.et al. (2011) Integrated genome-wide chromatin occupancy and expression analyses identify key myeloid pro-differentiation transcription factors repressed by Myb. Nucleic Acids Res.39: 4664–4679.

 
 

Tandon, P.et al. (2011) Requirement for ribosomal protein S6 kinase 1 to mediate glycolysis and apoptosis resistance induced by Pten deficiency. Proc. Natl. Acad. Sci. USA 108: 2361–2365.

 
 

Belzile, J-P. et al. (2010) HIV-1 Vpr induces the K48-linked polyubiquitination and proteasomal degradation of target cellular proteins to activate ATR andpPromote G2 arrest. J. Virol. 84: 3320 – 3330.

 
 

Jeong, S.M. et al. (2010) The SWI/SNF chromatin-remodeling complex modulates peripheral T cell activation and proliferation by controlling AP-1 expression. J. Biol. Chem. 285: 2340–2350.

 
 
神经生物学应用

Camilo, E. et al. (2011) Immune modulation effects of concomitant temozolomide and radiation therapy on peripheral blood mononuclear cells in patients with glioblastoma multiforme. Neuro. Oncol. 13: 393–400.

 
 

Ferrandi, C. et al. (2011) Characterization of immune cell subsets during the active phase of multiple sclerosis reveals disease and c-Jun N-terminal kinase pathway biomarkers. Mult. Scler. 17: 43–56.